Effect of different media, sugars, illumination and temperatures on the production of styrylpyrone derivatives in callus culture of Goniothalamus umbrosus

A.K. Mohamad Kamal, S. Subari, H.L.P. Azimahtol, R. Suri and H. Aminuddin

Abstract

Cell cultures of Goniothalamus umbrosus represent an alternative to whole plant extraction as a source of styrylpyrone derivatives (SPD). The aim of this study was to optimize the cell growth and production of SPD in callus culture of G. umbrosus. Growth was measured by fresh and dry weights of the calli and the content of SPD produced was determined by HPLC. The callus cultures of G. umbrosus were established on MS medium supplemented with 3% sucrose with the combination of hormone, 2.4-D (3.0 mg/litre) and BAP (0.5 mg/litre). The cultures were incubated at 25 ± 2 °C and under white light condition. Maximum growth of callus culture was 31.75 ± 2.25 g fw/culture and 4.73 ± 0.18 g dw/culture at 6 weeks of incubation. The SPD accumulation in callus varied from 0.041–1.96 µg/g dw sample.

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