Application of Trichoderma spp. to control stem end rot disease of mango var. Harumanis

A. Suhanna, Y. Norhanis and A. Hartinee

Abstract

A total of 47 isolates of Trichoderma spp. were isolated from soil at 8 different locations of mango farms for screening of their antagonistic effect against Botryodiploidia theobromae, the important postharvest disease of mangoes. The results showed that isolate T46 exhibited the greatest percentage inhibition of radial growth (PIRG) at day 6 (77.65%) while isolate T9 exhibited the greatest PIRG at day 9 (87.45%). Location significantly affected the soil pH and particle density which in turn influenced the percentage inhibition of Trichoderma spp. The application of isolate T9 was studied on mango Harumanis. The mangoes were sprayed with propiconazole at 500 ppm and 3 different spore concentrations of T9 at 1x103, 1x106 and 1x108 conidia/ml with and without inoculation of B. theobromae. Propiconazole was effective to inhibit the growth of B. theobromae in fruits inoculated with the pathogen which caused only 29.4% disease lesion compared with untreated mango (control) which had 70% disease lesion. Among the 3 spore concentrations used, 1x108 conidia/ml showed the lowest disease severity at 38.9%. All the fruits without inoculation with B. theobromae did not show any disease symptoms until day 4. Disease started to develop at day 6 for fruits sprayed with spore concentration 1x108 conidia/ml but other treatments were not significant. All treatments including control showed disease severity ranging from 0 – 1.67% at day 8.

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