Molecular typing among poultry isolates of Salmonella agona by PCR-based techniques and pulsed field gel electrophoresis (PFGE)

A.M. Sahilah, S. Radu, K.Y. Lum and L. Samuel

Abstract

Nine genomic DNA of Salmonella agona isolated from poultry sources were characterised by three variations of PCR-based techniques [polymerase chain reaction (PCR)-ribotyping, enterobacterial repetitive intergenic consensuspolymerase chain reaction (ERIC-PCR), and random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR)] and pulsed field gel electrophoresis (PFGE). These isolates originated from three different states of Peninsular Malaysia (Pulau Pinang, Negeri Sembilan and Selangor). The results of PCR-ribotyping, ERIC-PCR, RADP-PCR and PFGE to differentiate between nine isolates of S. agona were compared. The isolates were separated into three different genome types by PCR-ribotyping, nine and eight genome types by ERIC-PCR and PFGE, respectively.

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