Somaclonal variation in Chrysanthemum morifolium generated through petal cultures

P. Vilasini and Z. Latipah

Abstract

Ray florets of five varieties of Chrysanthemum morifolium were cultured in vitro and the regenerants were evaluated for somaclonal variation. The regenerated shoots exhibited variation in vegetative growth and flowering but limited floral variation. The technique used here could be used to secure naturally occurring colour mutants or sports. This was demonstrated when a yellow coloured floret was successfully isolated and regenerated to exhibit a solid mutant. Such mutants are impossible to isolate vegetatively due to its location and their limited extent.

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