Construction of cDNA libraries from ripe and unripe Eksotika 2 papaya

U. K. Abu Bakar and P. F. Lam

Abstract

To enable the isolation of useful genes involved in the ripening process of Eksotika 2 papaya, two cDNA libraries were constructed from the ripe and unripe papaya. Both libraries were constructed using the λZAP cDNA synthesis kit and the λZAP XR vectors. The green fruit library yielded recombinant phage of 7.5 x 104 pfu/mL which was amplified to 9.0 x 109 pfu/mL while the ripe fruit library yielded recombinant phage of 3.19 x 105 pfu/mL and amplified to 4.4 x 1010 pfu/mL. The ripe fruit library was successfully used for the isolation of several important genes from ripe Eksotika 2 papaya

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